Drug Absorption Studies: In Situ, In Vitro And In Silico Mod

Description:
This is a well thought-out, highly practical text covering contemporary 'in vitro' techniques for drug absorption studies. Starting at the molecular level of investigation, it continues with cell monolayer models (both primary and cell lines) and culminates with in situ techniques as a final testing format. In addition, chapters on high-throughput assays, in vitro-in vivo correlation, bioinformatics and regulatory issues are covered, giving a comprehensive overview of available models and techniques. Moreover, an appendix consisting of a number of practical protocols is available online, updated as needed, and should prove very helpful to apply the techniques directly to the benchside.

Table of Contents:
Perfused Organ Level/In Situ Techniques.- Models for skin absorption and skin toxicity testing.- Models of the small intestine.- Drug absorption from the colon in situ.- In vivo and ex vivo models for assessing drug delivery across the buccal mucosa.- In situ and ex vivo nasal models for preclinical drug development studies.- The isolated perfused lung for drug absorption studies.- Cellular Level -- In Vitro Models of Epithelial and Endothelial Barriers.- In vitro models for investigations of buccal drug permeation and metabolism.- In vitro screening models to assess intestinal drug absorption and metabolism.- In vitro cellular models for nasal drug absorption studies.- In vitro models of the bronchial epithelium.- In vitro models of the alveolar epithelial barrier.- Cell culture models of the corneal epithelium and reconstructed cornea equivalents for in vitro drug absorption studies.- The conjunctival barrier in ocular drug delivery.- Inner blood-retinal barrier: transport biology and methodology.- Regulation of paracellular permeability in low-resistance human vaginal-cervical epithelia.- In vitro models and multidrug resistance mechanisms of the placental barrier.- In vitro models to study blood-brain barrier function.- High-throughput screening using Caco-2 cell and PAMPA systems.- Instrumented approaches for the assessment of drug transport across intestinal and pulmonary epithelial barriers.- Bioinformatics -- In Silico Tools to Predict Drug Absorption.- Modelling transdermal absorption.- Physiologically based in silico models for the prediction of oral drug absorption.- In silico modeling for blood-brain barrier permeability predictions.- Molecular/Sub-Cellular Level -- Mechanistic Tools.- Impact of drug transport proteins.- Cloning and functional heterologous expression of transporters.- Pharmacology of caveolae.- Immortalization strategies for epithelial/endothelial cells in primary culture.- Binding-uptake studies -- cellular targeting.- Drug permeability studies in regulatory biowaiver applications.